Wells Fargo & Co., citing credit events after its Jan. 28 report, added a pretax $328.4 million impairment of perpetual preferred securities to its fourth-quarter loss.
Shares fell 3.2% to $16.30 in after-hours trading.
As a result of the charge, the bank's quarterly loss is now $2.73 billion, or 84 cents a share, compared with the previously reported $2.55 billion, or 79 cents a share.
That was the first quarterly loss for San Francisco-based Wells Fargo since 2001. Charlotte-based Wachovia Corp., which wasn't included in the results because Wells didn't close on the purchase until Dec. 31, lost $11.2 billion during the period.
Because the securities were carried at fair value as of Dec. 31, the loss on the securities was previously reported as unrealized losses on securities available for sale.
Like other large lenders, Wells continues to reel from the effects of declines in residential real-estate values, higher unemployment rates and more borrower bankruptcies.
Some analysts have said Wells will need to cut its dividend and raise even more capital to account for Wachovia's problem loans and boost its balance sheet, but Wells so far has made no moves on either front, maintaining its dividend at 34 cents a share and declaring it has "no plans" to request more government aid. It received $25 billion from the government last year and raised an additional $13 billion in a common-share offering.